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ISSN Online: 2377-424X

ISBN Print: 0-89116-909-1

International Heat Transfer Conference 9
August, 19-24, 1990 , Jerusalem, Israel

A STUDY ON FREEZING PROCESS OF A TERNARY SYSTEM USED FOR PRESERVATION OF BIOLOGICAL CELLS

Get access (open in a dialog) DOI: 10.1615/IHTC9.3730
pages 79-84

Résumé

In the freezing preservation of biological cells, the cells are often suspended inside physiological solution. The cryoprotective agent (CPA) has to be put into the solution to minimize the cell's cryoinjury which is caused by the freezing process itself. The mechanism of the function of CPA against the cryoinjury has not been known clearly. In present paper, a heat transfer model is developed to study the effect of the initial concentration of glycerol (one of CPAs) on freezing process of a physiological ternary-system, glycerol-NaCl-water, taking place in a cylindrical tube.
From the numerical and experimental investigation, it was found that (1) the initial concentration of glycerol greatly affected the transient freezing process of the ternary system; (2) the glycerol reduced the electrolyte concentration in the unfrozen solution at any subzero temperature at which the solution was kept. The higher the initial glycerol concentration, the more was the electrolyte concentration reduced; (3) the glycerol prevented the frozen solution from crush; (4) it may be predicted that the glycerol contributes to reduce the cryoinjury of the biological cells by, at least, minimizing the "Solution Effect" and the "Thermal Stress Effect" on the cells in the cryopreservation.