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ISSN Online: 2377-424X

International Heat Transfer Conference 12
August, 18-23, 2002, Grenoble, France

Three-Dimensional Behavior of Ice Crystals and Cells During Freezing and Thawing of Biological Tissues

Get access (open in a dialog) DOI: 10.1615/IHTC12.2280
6 pages

Résumé

Three-dimensional behavior of ice crystals and cells during the freezing and thawing of biological tissues was investigated microscopically in time-series by using a confocal laser scanning microscope (CLSM) and a fluorescent dye, acridine orange (AO). Fresh tender meat (the second pectoral muscles) of chicken was stained with the AO in physiological saline, and then frozen and thawed in a uniform temperature under two different thermal protocols: a) slow-cooling and slow-warming and b) rapid-cooling and slow-warming. The CLSM noninvasively produced tomograms of the tissues to clarify the pattern of freezing, morphologies of extra- and intracellular ice crystals and cells in the tissues, and the interaction between ice crystals and cells. After freezing and thawing, the tissues were also investigated morphologically by histological means. The histological changes of tissues were compared between the thermal protocols and correlated with the results from time-series 3D visualization by the CLSM/dye method.