A thermoacoustic refrigerator produces heat transfer potential from acoustic power through the use of a resonator, a loudspeaker, and a porous stack. A standing wave is formed within the resonator at a specific frequency, which facilitates the desired air particle movement. The interaction of the moving particles with the stack then enables the transfer of heat from one side of the resonator to the other. The subsequent placement of heat exchangers on either side of the stack allows heat transfer to/from thermal reservoirs external to the device. In this study, a thermoacoustic refrigeration prototype was built and evaluated through various experimental phases and by comparison to DeltaEC - a thermoacoustic refrigeration modelling tool. The intention was to determine the feasibility of the prototype for a specific commercial application. Interest was placed on the mobility and low-cost nature of the device for a low load-based application - more specifically the treatment of therapeutic hypothermia within an ambulance or transport setting. The experiment tested the response of the system at various operating conditions by changing the electrical power input, the mean pressure, and a simulated environmental temperature. The acoustic aspects of the model were well validated. The simulated thermal behaviour of the system could not be achieved in practice due to the constraints imposed by the real heat exchanger performance. Thermoacoustic research should thus be directed at the effective modelling, design, and implementation of practical heat exchangers within real-world thermoacoustic systems.